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This Concept Map, created with IHMC CmapTools, has information related to: LAB 13 - ISOLATION AND IDENTIFICATION OF ENTEROBACTERIACEAE AND PSEUDOMONAS, PART 2, Enterobacter and Klebsiella appearance Weak fermentation of lactose by the bacteria causes the colonies and confluent growth to appear pink to red, but there is no pink precipitate (cloudiness) in the agar surrounding the growth, Gibson Oxidase test oxidase negative = usually fermentative (Enterobacteriaceae) Inoculate a MacConkey Agar plate and an Enteropluri-Test. Incubate at 37C., XLD agar: Selective for Gram-negative bacteria. Different Gram-negative bacilli, due to their biochemical reactions, produce different appearing colonies. appearance phenol red turns yellow somewhere on the XLD agar (yellow colonies), phenol red turns yellow somewhere on the XLD agar (yellow colonies) possible unknowns Escherichia, Enterobacter, Klebsiella, or Proteus, phenol red turns a deeper red with no yellow on the XLD agar; colonies do not have black centers (red colonies without black centers) possible unknowns Shigella or Proteus, Inoculate a MacConkey Agar plate and an Enteropluri-Test. Incubate at 37C. examples Enterobacter and Klebsiella, See section C of your lab manual, IDENTIFYING MEMBERS OF THE ENTEROBACTERIACEAE WITH THE ENTEROPLURI-TEST explaination of test result Interpretation of the Enteropluri-Test (You do not have to know the results of these of the Enteropluri-tests, just be able to use the Enterpluri-test given the tables in your lab manual.), the Enteropluri-Test: A self-contained, compartmented plastic tube containing 12 different agars, enabling the performance of a total of 15 standard biochemical tests. identification See section C of your lab manual, IDENTIFYING MEMBERS OF THE ENTEROBACTERIACEAE WITH THE ENTEROPLURI-TEST, Inoculate an XLD agar plate and an Enteropluri-Test. Incubate at 37C. results Enterobacteriaceae, Case Study #1 Gram stain Gram-negative bacilli, Gram-negative bacilli fermentative or non-fermentative Gram-negative bacillus Gibson Oxidase test, Pseudomonas isolation Cetrimide agar: Selective for Pseudomonas aeruginosa and stimulates P. aeruginosa to produce its characteristic pigment as well as fluorescent products., Escherichia coli appearance The colonies and confluent growth to appear bright pink to red with a pink precipitate (cloudiness) appearing in the agar surrounding the growth, Pseudomonas identification pigment production and fluorescence, Gibson Oxidase test identification ????, odor results P. aeruginosa produces a characteristic fruity or grape juice-like aroma due to production of an aromatic compound called aminoacetophenone. Most of the Enterobacteriaceae smell rather foul., oxidase test results P.aeruginosa and most other non-fermentative gram-negative rods are oxidase-positive; most Enterobacteriaceae are oxidase-negative., Inoculate a Pseudosel agar plate and an Enterotube® II. Incubate at 37C. results Pseudomonas, pigment production and fluorescence results P. aeruginosa produces a green to blue, water-soluble pigment called pyocyanin, as well as fluorescein, a compound that will fluoresce under short wavelength ultraviolet light. The Enterobacteriaceae do not produce pigment at 37C and do not fluoresce., XLD agar: Selective for Gram-negative bacteria. Different Gram-negative bacilli, due to their biochemical reactions, produce different appearing colonies. appearance phenol red turns a deeper red with no yellow on the XLD agar; come colonies have black centers (red colonies with black centers)
