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This Concept Map, created with IHMC CmapTools, has information related to: LAB 12 - ISOLATION AND IDENTIFICATION OF ENTEROBACTERIACEAE AND PSEUDOMONAS, PART 1, Pseudomonas causes opportunistic infections wound infections, Pseudomonas identification fermentation of glucose, Shigella appearance red colonies without black centers, odor results P. aeruginosa produces a characteristic fruity or grape juice-like aroma due to production of an aromatic compound called aminoacetophenone. Most of the Enterobacteriaceae smell rather foul., gastrointestinal (gi) pathogens medically-important genera Salmonella, MacConkey Agar: A selective medium used for the isolation of non-fastidious Gram-negative rods, particularly members of the family Enterobacteriaceae and the genus Pseudomonas, and the differentiation of lactose fermenting from lactose non-fermenting Gram-negative bacilli. MacConkey agar contains the dye crystal violet well as bile salts that inhibit the growth of most Gram-positive bacteria but do not affect the growth of most Gram-negatives. If the Gram-negative bacterium ferments the sugar lactose in the medium, the acid end products lower the pH of the medium. The neutral red in the agar turns red in color once the pH drops below 6.8. As the pH drops, the neutral red is absorbed by the bacteria, causing the colonies to appear bright pink to red. examples Escherichia coli, gastrointestinal (gi) pathogens medically-important genera Shigella, XLD agar Selective for Gram-negative enteric bacteria, especially intestinal pathogens such as Salmonella and Shigella. it detects fermentation of the sugars lactose and sucrose, dercarboxylation of the amino acid L-lysine, and the production of hydrogen sulfide. examples Salmonella, MacConkey Agar: A selective medium used for the isolation of non-fastidious Gram-negative rods, particularly members of the family Enterobacteriaceae and the genus Pseudomonas, and the differentiation of lactose fermenting from lactose non-fermenting Gram-negative bacilli. MacConkey agar contains the dye crystal violet well as bile salts that inhibit the growth of most Gram-positive bacteria but do not affect the growth of most Gram-negatives. If the Gram-negative bacterium ferments the sugar lactose in the medium, the acid end products lower the pH of the medium. The neutral red in the agar turns red in color once the pH drops below 6.8. As the pH drops, the neutral red is absorbed by the bacteria, causing the colonies to appear bright pink to red. examples Salmonella, Shigella, Proteus, Serratia, and Pseudomonas, opportunistic pathogens medically-important genera Citrobacter, cause opportunistic infections pneumonia, Pseudomonas causes opportunistic infections urinary tract infections, Enterobacteriaceae isolation on XLD Agar XLD agar Selective for Gram-negative enteric bacteria, especially intestinal pathogens such as Salmonella and Shigella. it detects fermentation of the sugars lactose and sucrose, dercarboxylation of the amino acid L-lysine, and the production of hydrogen sulfide., Enterobacteriaceae isolation on MacConkey Agar MacConkey Agar: A selective medium used for the isolation of non-fastidious Gram-negative rods, particularly members of the family Enterobacteriaceae and the genus Pseudomonas, and the differentiation of lactose fermenting from lactose non-fermenting Gram-negative bacilli. MacConkey agar contains the dye crystal violet well as bile salts that inhibit the growth of most Gram-positive bacteria but do not affect the growth of most Gram-negatives. If the Gram-negative bacterium ferments the sugar lactose in the medium, the acid end products lower the pH of the medium. The neutral red in the agar turns red in color once the pH drops below 6.8. As the pH drops, the neutral red is absorbed by the bacteria, causing the colonies to appear bright pink to red., gastrointestinal (gi) pathogens transmitted by fecal-oral route, Shigella causes shigellosis (gi infection), Salmonella appearance usually red colonies with black centers, certain strains of Escherichia coli: ETEC, EPEC, EAEC, EIEC, STEC causes gi infections, gastrointestinal (gi) pathogens medically-important genera certain strains of Escherichia coli: ETEC, EPEC, EAEC, EIEC, STEC, Pseudomonas causes opportunistic infections septicemia
