Across
- An acidic dye will give a ___________ stain.
- Dye in which the color portion resides in the positive ion.
- A bacterial cell wall containing only 2-3 layers of peptidoglycan and surrounded by an outer membrane composed of phospholipids, lipopolysaccharide, lipoprotein, and proteins. (2 words)
- During starch hydrolysis, starch is broken down to __________.
- Secreted by bacteria into the surrounding environment in order to break down larger nutrient molecules so they may enter the bacterium.
- Dye in which the color portion resides in the negative ion.
- Gram-negative bacteria stain _______ after gram staining.
- A basic dye will give a ___________ stain.
- An exoenzyme that hydrolizes a protein is called a _____________.
- The emergence of a single vegetative bacterium from an endospore.
- If SIM medium turns black after inoculation and incubation, what has the bacterium produced? (2 words)
- This structure helps many pathogenic and normal flora bacteria to initially resist phagocytosis by the host's phagocytic cells. In soil and water, capsules help prevent bacteria from being engulfed by protozoans. It also helps many bacteria to adhere to surfaces and thus resist flushing.
- A single flagellum at one pole.
- The second reagent used during gram staining is__________. (2 words)
- Two or more flagella at one or both poles of the cell.
- Growth along the stab line in Motility Test medium appears cloud-like or "out of focus" as it moves away from the stab line. The bacterium is _____________.
- The third reagent used during gram staining is__________. (2 words)
- The principle protein found in milk that gives milk its white, opaque appearance.
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Down
- A broad, dense bacterial cell wall 20-80 nm thick and consisting of numerous interconnecting layers of peptidoglycan. Interwoven in the cell wall are teichoic acids. (2 words)
- An exoenzyme that hydrolizes starch is called a __________.
- The fortht reagent used during gram staining is__________.
- In carbohydrate fermentation, the Durham tube is used to detect the production of _________.
- During protein hydrolysis, proteins are broken down into ______________. (2 words)
- An anaerobic process during which carbohydrates are broken down for energy production.
- Gram-positive bacteria stain _______ after gram staining.
- Axial filaments are iInternal flagella found only in __________.
- Completely surrounded by flagella.
- A single flagellum at both poles.
- A genus of bacterium that produces endospores.
- A microscope that uses special optics convert slight variations in specimen thickness into corresponding visible variation in brightness. Thus, the bacterium and its structures appear darker than the background. (2 words)
- Used inside the bacterium, to brake down nutrients to yield energy for driving various cellular functions or to form building blocks to synthesize cellular components.
- A microscope that uses a special condenser to direct light away from the objective lens. However, bacteria lying in the transparent medium will scatter light so that it enters the objective. The organism will appear bright against the dark background.
- If the Kovac's reagent added to SIM medium after inoculation and incubation turns red, what has the bacterium produced?
- If no fermentation occurs, phenol red lactose broth will appear ________.
- In preparing slides for staining, what is used to stick the bacteria to the glass so they do not wash off?
- The first reagent used during gram staining is__________. (2 words)
- A resistant, dormant survival form of certain bacteria that is resistant to heat, drying, radiation, and various chemical disinfectants.
- Growth occurs only along the line of inoculation in Motility Test medium. Growth along the stab line is very sharp and defined. The bacterium is ________________.
- The enzyme that breaks hydrogen peroxide down into water and oxygen.
- Another word for the bacterial capsule.
- If lactose is fermented by a bacterium, the phenol red lactose broth will appear ________.
- Fermentation of carbohydrates by bacteria always produces _________ end products.
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