Direct Fluorescent Antibody Technique:
Negative Results
The unknown antigen is fixed to a slide. Known antibodies against the suspected
antigen are added. The slide is then washed and viewed under a fluorescent microscope.
If the known antibodies with attached fluorescent dye do not correspond to the
suspected antigen, they will wash off the slide and the antigen will not fluoresce
when viewed under a fluorescent microscope.
