Rotate the plate so that area "2" is on your left. Drag your sterile inoculating loop through area "2" two times and spread the inoculum on area "2"
over area "3." Sterilize the loop.

Microbiology Laboratory Manual by Gary E. Kaiser, PhD, Professor of Microbiology
is licensed under a Creative Commons Attribution 4.0 International License.
Last updated: August, 2017