The leading strand is made continuously in a 5' to 3' direction by DNA polymerase III as the DNA helicase unwinds the parental DNA helix. However, because the parental DNA strands are antiparallel, the lagging strand must be made in short fragments. RNA polymerase (primase) synthesizes a short RNA primer which is extended by DNA polymerase III. DNA polymerase II then digests the RNA primer and replaces it with DNA. Finally, DNA ligase joins the fragments of the lagging strand together.

by Gary E. Kaiser, Ph.D.
Professor of Microbiology, The Community College of Baltimore County, Catonsville Campus
This work is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work The Grapes of Staph at https://cwoer.ccbcmd.edu/science/microbiology/index_gos.html.