The Gram-Positive Cell Wall (def)
As mentioned in the previous section on peptidoglycan, Gram-positive bacteria are those that retain the initial dye crystal violet during the Gram stain procedure and appear purple when observed through the microscope. As we will learn in lab, this is a result of the structure and function of the Gram-positive cell wall.
Common Gram-positive bacteria of medical importance include Streptococcus pyogenes, Streptococcus pneumoniae, Staphylococcus aureus, Enterococcus faecalis, and Clostridium species.
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A. Structure and Composition of the Gram-Positive Cell Wall
1. In electron micrographs, the Gram-positive cell wall appears as a broad, dense wall 20-80 nm thick and consisting of numerous interconnecting layers of peptidoglycan (see Figs. 1A and 1B). Chemically, 60 to 90% of the Gram-positive cell wall is peptidoglycan. In Gram-positive bacteria it is thought that the peptidoglycan is laid down in cables of several cross-linked glycan strands approximately 50 nm wide. These cables then themselves become cross-linked for further cell wall strength.
2. Interwoven in the cell wall of Gram-positive are teichoic acids and lipoteichoic acids. Teichoic acids extend through and beyond the rest of the cell wall and are polyalcohols composed of polymers of glycerol, phosphates, and the sugar alcohol ribitol and are covalently bound to the peptidoglycan. Teichoic acids covalently bound to cytoplasmic membrane lipids are called lipoteichoic acids (see Fig. 1B).
3. The outer surface of the peptidoglycan is studded with surface proteins that differ with the strain and species of the bacterium (see Fig. 1B).
4. The periplasm (def) is the gelatinous material between the peptidoglycan and the cytoplasmic membrane.
B. Functions of the Gram-Positive Cell Wall Components
1. The peptidoglycan in the Gram-positive cell wall prevents osmotic lysis (def).
2. The teichoic acids probably help make the cell wall stronger (see Fig. 1B).
3. The surface proteins (see Fig. 1B) in the bacterial peptidoglycan, depending on the strain and species, carry out a variety of activities.
a. Some surface proteins function as enzymes.
b. Other proteins serve as adhesins (def). Adhesins enable the bacterium to adhere intimately to host calls and other surfaces in order to colonize those cells and resist flushing (See Fig. 2 ).
by Gary E. Kaiser, Ph.D.
Professor of Microbiology, The Community College of Baltimore County, Catonsville Campus
This work is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work The Grapes of Staph at https://cwoer.ccbcmd.edu/science/microbiology/index_gos.html.Last updated: August, 2019
Please send comments and inquiries to Dr. Gary Kaiserc. Many bacteria involved in infection have the ability to co-opt the functions of host cells for the bacterium's own benefit. This is done by way of bacterial secretions systems that enable the bacterium to directly inject bacterial effector molecules into the cytoplasm of the host cell in order to alter its cellular machinery or cellular communication to the benefit of the bacteria. They do this by producing secretion systems such as the type 3 secretion system that produces hollow, needle-like tubes called injectisomes (def). Certain bacteria, for example, inject invasins (def) into the cytoplasm of the host cell that enable the bacterium to enter that cell.
by Gary E. Kaiser, Ph.D.
Professor of Microbiology, The Community College of Baltimore County, Catonsville Campus
This work is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work The Grapes of Staph at https://cwoer.ccbcmd.edu/science/microbiology/index_gos.html.Last updated: August, 2019
Please send comments and inquiries to Dr. Gary KaiserThe role of these cell wall surface proteins will be discussed in greater detail later in Unit 3 under Bacterial Pathogenicity.4. The periplasm (def) contains enzymes for nutrient breakdown.
C. Significance of Gram-Positive Cell Wall Components to the Initiation of Body Defenses
The body has two immune systems: the innate immune system and the adaptive immune system.
1. Innate immunity is an antigen-nonspecific (def) defense mechanisms that a host uses immediately or within several hours after exposure to almost any microbe. This is the immunity one is born with and is the initial response by the body to eliminate microbes and prevent infection.
2. Adaptive (acquired) immunity refers to antigen-specific (def) defense mechanisms that take several days to become protective and are designed to react with and remove a specific antigen. This is the immunity one develops throughout life.
Initiation of Innate Immunity
In order to protect against infection, one of the things the body must initially do is detect the presence of microorganisms. The body does this by recognizing molecules unique to microorganisms that are not associated with human cells. These unique molecules are called pathogen-associated molecular patterns or PAMPs (def). (Because all microbes, not just pathogenic microbes, possess PAMPs, pathogen-associated molecular patterns are sometime referred to as microbe-associated molecular patterns or MAMPs.)
Fragments of peptidoglycan and teichoic acids are PAMPS associated with the cell wall of Gram-positive bacteria. In addition, bacteria and other microorganisms also possess mannose-rich glycans (short carbohydrate chains with the sugar mannose or fructose as the terminal sugar) that function as PAMPs. These mannose-rich glycans are common in microbial glycoproteins and glycolipids but rare in those of humans (see Fig. 3).
These PAMPS bind to pattern-recognition receptors or PRRs (def) on a variety of defense cells of the body and trigger such innate immune defenses as inflammation (def), fever, and phagocytosis.
Inflammation (def) is the first response to infection and injury and is critical to body defense. Basically, the inflammatory response is an attempt by the body to restore and maintain homeostasis (def) after injury. Most of the body defense elements are located in the blood, and inflammation is the means by which body defense cells and body defense chemicals leave the blood and enter the tissue around an injured or infected site.
Body defense cells such as macrophages (def), and dendritic cells (def) have pattern recognition receptors such as toll-like receptors on their surface that are specific for the peptidoglycan fragments and lipoteichoic acids in the Gram-positive cell wall and/or to NODs in their cytoplasm that are specific for peptidoglycan fragments.
The binding of these cell wall components to their corresponding pattern recognition receptors triggers the macrophages to release various defense regulatory chemicals called cytokines (def), including IL-1, IL-6, IL-8, TNF-alpha, and PAF. The cytokines then bind to cytokine receptors on target cells and initiate inflammation and activate both the complement pathways (def) and the coagulation pathway (see Fig. 4).
The peptidoglycan and teichoic acids also activate the alternative complement pathway and the lectin pathway (def), innate immune defense pathways that play a variety of roles in body defense.
Innate immunity will be discussed in greater detail in Unit 5.
Initiation of Adaptive Immunity
Proteins and polysaccharides associated with the Gram-positive cell wall function as antigens and initiate adaptive immunity. An antigen (def) is defined as a molecular shape that reacts with antibody molecules and with antigen receptors on lymphocytes. We recognize those molecular shapes as foreign or different from our body's molecular shapes because they fit specific antigen receptors on our B-lymphocytes and T-lymphocytes, the cells that carry out adaptive immunity.
The actual portions or fragments of an antigen that react with antibodies and with receptors on B-lymphocytes and T-lymphocytes are called epitopes (def). An epitope is typically a group of 5-15 amino acids with a unique shape that makes up a portion of a protein antigen, or 3-4 sugar residues branching off of a polysaccharide antigen. A single microorganism has many hundreds of different shaped epitopes that our lymphocytes can recognize as foreign and mount an adaptive immune response against.
The body recognizes an antigen as foreign when epitopes of that antigen bind to B-lymphocytes (def) and T-lymphocytes (def) by means of epitope-specific receptor molecules having a shape complementary to that of the epitope. The epitope receptor on the surface of a B-lymphocyte is called a B-cell receptor and is actually an antibody molecule. The receptor on a T-lymphocyte is called a T-cell receptor (TCR).
There are two major branches of the adaptive immune responses: humoral immunity and cell-mediated immunity.
1. Humoral immunity (def): Humoral immunity involves the production of antibody molecules in response to an antigen (def) and is mediated by B-lymphocytes. Through a variety of mechanisms, these antibodies are able to remove or neutralize microorganisms and their toxins after binding to their epitopes. For example, antibodies made against cell wall antigens can stick bacteria to phagocytes, a process called opsonization (def). Antibodies made against cell wall adhesins can prevent bacteria from adhering to and colonizing host cells.
by Gary E. Kaiser, Ph.D.
Professor of Microbiology, The Community College of Baltimore County, Catonsville Campus
This work is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work The Grapes of Staph at https://cwoer.ccbcmd.edu/science/microbiology/index_gos.html.Last updated: August, 2019
Please send comments and inquiries to Dr. Gary Kaiser
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by Gary E. Kaiser, Ph.D.
Professor of Microbiology, The Community College of Baltimore County, Catonsville Campus
This work is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work The Grapes of Staph at https://cwoer.ccbcmd.edu/science/microbiology/index_gos.html.Last updated: August, 2019
Please send comments and inquiries to Dr. Gary Kaiser2. Cell-mediated immunity (def): Cell-mediated immunity involves the production of cytotoxic T-lymphocytes, activated macrophages, activated NK cells, and cytokines in response to an antigen (def) and is mediated by T-lymphocytes. These defense cells help to remove infected cells and cancer cells displaying foreign epitopes.
Adaptive immunity will be discussed in greater detail in Unit 6.
D. Significance of Gram-Positive Cell Wall Components to Bacterial Pathogenicity
During severe systemic infections (def) with large numbers of bacteria present, however, high levels of Gram-positive PAMPs are released resulting in excessive cytokine production by the macrophages and other cells and this, in turn, can harm the body (see Fig. 5).
by Gary E. Kaiser, Ph.D.
Professor of Microbiology, The Community College of Baltimore County, Catonsville Campus
This work is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work The Grapes of Staph at https://cwoer.ccbcmd.edu/science/microbiology/index_gos.html.Last updated: August, 2019
Please send comments and inquiries to Dr. Gary Kaiser
Medscape article on infections associated with organisms mentioned in this Learning Object. Registration to access this website is free.